Fig. 3
From: PcTrim prevents early infection with white spot syndrome virus by inhibiting AP1-induced endocytosis
PcTrim inhibits WSSV endocytosis in crayfish. The subcellular localization of PcTrim was detected using an immunocytochemical assay (A) and western blot (B). Dil was used to label the cell membrane, and DAPI was used to label the cell nuclei. The role of PcTrim in phagocytosis was studied by analyzing the phagocytosis rate of FITC-labeled VP28. After RNAi of Trim, FITC-labeled VP28 was injected into crayfish. Hemocytes were collected 30 min after VP28 injection (C), and phagocytosis of VP28 by hemocytes was observed via microscopy. D The phagocytosis rate after Trim-RNAi treatment was calculated using the described formula. dsGFP was used as a control. E After blocking Trim via injection with anti-Trim antibody, the rate of VP28 phagocytosis by hemocytes was analyzed with a fluorescence microscope. F The phagocytosis rate after Trim blocked. Anti-actin was used as a control. G Hemocyte phagocytosis observed under a fluorescence microscope after rTrim injection. H The phagocytosis rate after His-Trim injection. The asterisk indicates a significant difference, p < 0.05