Fig. 2
From: PcTrim prevents early infection with white spot syndrome virus by inhibiting AP1-induced endocytosis
PcTrim interacts with VP26. Far western blot was used to screen the interaction between VPs and Trim. Lysates of gills were incubated with His resin containing His-Trim (A, B, C), and then, three antibodies (anti-VP24, anti-VP26, and anti-VP28) were used to detect the presence of VP24, VP26 and VP28. His-Tag was used as negative control. The β-Actin was used as inner protein control. VP24, VP26 and VP28 were detected in each samples. Only VP26 was detected to interact with His-Trim (B). A pull-down assay was used to analyze the interaction between Trim and VP26. His-Trim was first bound to His-resin, and then, the resin was incubated with GST-VP26. After 3 washes with PBS, protein residues were analyzed via SDS‒PAGE (D). The interaction between GST-VP26 and His-Trim (E) or between GST-VP26 and His-Tag (F) was analyzed using the above methods with GST resin or His resin. The co-IP assay was performed using anti-PcTrim and anti-VP26 antibodies and gill lysates from WSSV-infected crayfish. A tube with antiserum-free protein A resin was used as a control (G)