Fig. 1
From: PcTrim prevents early infection with white spot syndrome virus by inhibiting AP1-induced endocytosis
PcTrim inhibits WSSV replication. A Tissue distribution of PcTrim in crayfish at mRNA level. B–D Expression patterns of PcTrim in hemocytes (B), hepatopancreas (C) and the stomach (D), as detected by qRT‒PCR. 18S rRNA was used as the control. qRT‒PCR and western blotting were used to analyze the amounts of WSSV (vp28 was used as a marker of WSSV copy number). The expression levels of VP28 were analyzed in His-Trim injection crayfish (E, J), anti-Trim antibody injection crayfish (F, K) and dsTrim injection crayfish (H, N), and His-Tag, anti-Actin antibody and dsGFP were used as controls. The expression level of PcTrim was measured by qRT‒PCR (G) and western blotting (M) 48 h after dsTrim injection. I Recombinant expression and purification of PcTrim in E.coli. Lane 1, total proteins from E.coli with PcTrim-pET-32a, without ITPG induction; Lane 2, total proteins from E.coli with PcTrim-pET-32a, with ITPG induction; Lane 3, purified recombinant PcTrim. L PcTrim was detected using the PcTrim polyclonal antibodies. Crayfish were divided into two groups: the groups were injected with either His-Tag or His-Trim, and then, all the crayfish were injected with WSSV (O); two groups of crayfish were injected with either dsGFP or dsTrim, and then, the crayfish were challenged with WSSV after PcTrim-RNAi treatment (P). The survival rate of crayfish was calculated. The asterisk indicates a significant difference, p < 0.05