Fig. 8
From: BMAL1/p53 mediating bronchial epithelial cell autophagy contributes to PM2.5-aggravated asthma
p53 siRNA prevented PM2.5/carbon particle-induced collagen-I expression and cell autophagy in bronchial epithelial cells. HBEs were incubated for 48 h after transfection with control or specific siRNA against p53 and treated with carbon particles (20 μg/ml) or PM2.5 (200 μg/ml) for 48 h prior to harvest of cell lysates, after which expression levels of protein were measured by qRT-PCR and Western blots. A, B mRNA changes of collagen-I, α-SMA and MUC5AC in HBEs. n = 6. C, E Representative images of Western blots of p53, fibronectin, collagen-I, α-SMA and LC3B-II were shown. D, F The changes in relative density of p53, fibronectin, collagen-I, α-SMA and LC3B-II to GAPDH were presented. n = 3 (C); n = 6 (p53 and collagen-I in D), n = 4 (fibronectin, α-SMA and LC3B-II in D). Data are mean ± SEM of n individual experiments. *P < 0.05, **P < 0.01, ***P < 0.001 between indicated conditions (One-way ANOVA followed by the Bonferroni’s test)