Fig. 7
From: BMAL1/p53 mediating bronchial epithelial cell autophagy contributes to PM2.5-aggravated asthma
BMAL1 interacted with p53 via particular structural domains. A The strategy for Flag-p53 and HA-BMAL1 truncations. Different structural domains were represented by different color blocks, the specific details have been marked in the Fig. PRD, proline-rich domain; DBD, DNA binding domain; TD, tetramerization domain; RD, regulatory domain; HLH, helix-loop-helix domain; PAS: PER-ARNT-SIM domain. B, C Determination of p53 and BMAL1 domains required for their interaction with each other. Different p53-truncated constructs were co-transfected with HA-tagged BMAL1 and different BMAL1-truncated constructs were co-transfected with Flag-tagged p53 into HEK293T cells. The cells were then subjected to co-immunoprecipitation and Western blot analyses with the indicated antibodies