Fig. 2

Carbon particles and PM2.5 induced BMAL1 down-regulation in vitro and in vivo. A, C HBE cells were treated with carbon particles (20 μg/ml) or PM2.5 (200 μg/ml) for 24 h. Whole-cell protein lysates were prepared for Western blot analysis using anti-BMAL1, n = 3. B, D In the mouse asthma model induced by OVA and carbon particles or PM2.5, BMAL1 protein contents were measured by western blotting, n = 5 (B), n = 4 (D). Data are mean ± SEM of n individual experiments. *P < 0.05, **P < 0.01, ***P < 0.001. P values were determined by Student’s t test (A, C) or one-way ANOVA followed by the Dunnett’s test (B, D)