Fig. 6

Effect of curcumin treatment on mice splenocytes in vitro. CIA mice splenocytes were stimulated with LPS in the presence of curcumin and/or compound C for 3 days and then analyzed by flow cytometry. A Western blots of SMILE expression in the splenocytes of curcumin (25 μM)-treated mice administered LPS (100 ng/mL) and untreated mice after 6 h. The SMILE expression was elevated in curcumin treated cells. B Expression of BAFF-R- and CD19-positive cells. Both cells were decreased by curcumin treatment, but curcumin had no effect in the group treated with the AMPK inhibitor compound C. C Proportion of BAFF-R⁺ B cells (CD19⁺ BAFF-R⁺), IL-17 secreting B cells (CD19⁺ IL-17⁺), plasma cells (B220⁻ CD138⁺), and GC B cells (B220⁺ GL-7⁺) after curcumin treatment. *P < 0.05 and **P < 0.01