Fig. 6

DNMT1-mediated demethylation of SHP-1 was involved in the inhibition of Baicalein. A The extent of SHP-1 methylation was determined by MSP using methylation‑specific primers. U, unmethylated‑specific primers; M, methylated‑specific primers. SHP-1 methylation was determined by PCR agarose gel electrophoresis after treatment with decitabine. B Effects of Baicalein on SHP-1 methylation were evaluated by MSP, after treatment with or without decitabine. C K562 CD34⁺ cells were treated with 20 μM Baicalein for 36 h in the supernatant of hBMSCs. The methylation status of SHP-1 was analyzed by BSP. Black and white dots represented methylated and unmethylated CpG dinucleotides, respectively. DThe structure of Baicalein. E Docking result of the ligand Baicalein and DNMT1 was showed in 3D structure. Baicalein formed 2 hydrogen bonds (shown in green dashed lines) with DNMT1 and the -CDOCKER interaction energy was 46.5. F Docking result of the ligand Baicalein and DNMT1 was showed in 2D structure. Two hydrogen bonds were formed between Baicalein and DNMT1 as shown in green dashed lines. The pink dashed lines was shown as the aromatic stacking of Pi-alkyl. Viridescence was indicated as Van der Waals forces. Magenta dashed lines was shown as pi-pi stacking interaction