Fig. 5

The effect of Baicalein on DNMT1-mediated SHP-1 within BM microenvironment. A, B Effects of Baicalein on GM-CSF secretion in K562 CD34⁺ cells and primary CD34⁺ CML cells were detected by ELISA assay, respectively. C, D Effects of Baicalein on DNMT1 and SHP-1 expression were determined in both CML CD34⁺ cells. **p < 0.01, ***p < 0.001 versus control group (without Baicalein treatment). E, F The expressions of SHP-1 were determined, after treatment with 10 μM DNMT1 inhibitor(decitabine) in CML CD34⁺ cells. *p < 0.05, **p < 0.01, ***p < 0.001 versus control group, Data points of 3 independent experiments are depicted. G pCMV6-Entry shp-1 and pCMV6-Entry vector were transfected into CML CD34⁺ cells. Subsequently, apoptosis was measured by Annexin V-PI double staining assay after treatment with IM in co-culture model. H, I After transfection, the growth inhibition effect of IM on CD34⁺ subpopulation in K562 cells or primary CML cells with or without the supernatant of hBMSCs was detected by MTT. J, K Silence of SHP-1 by SHP-1shRNA reversed the effects of Baicalein on p-JAK2^Tyr1007/1008 and p-STAT5 ^Tyr694 (*p < 0.05, **p < 0.01, ***p < 0.001)