Table 1 CNS cell markers detected by proteomic analysis of the release method EVs divided into four cell-origin categories (neurons, microglia, astrocytes, oligodendrocytes)

CNS cell markers

Abcb1a, Acadl, Acp1, Add2, Agap2, Agpat3, Ahcyl2, Aifm3, Ak1, Alcam, Aldh5a1, Aldoc, Amph, Ank3, Anks1b, Anxa5, Arhgef9, Arl8a, Arpc5, Ass1, Atp1a3, Atp1b1, Atp2b4, Atp6v1g2, Atxn2, Bcan, Bcat1, Bin1, Brsk2, Cacna1e, Cacna2d1, Camk2a, Camk2b, Camk2g, Cap2, Capn2, Capn5, Carhsp1, Cat, Cd200, Cd47, Cd63, Cdk14, Cds1, Cept1, Chmp1a, Chordc1, Cit, Ckmt1, Clec2l, Clu, Cmtm4, Cnksr2, Cntnap1, Copb2, Cotl1, Cpne5, Cpne6, Crbn, Crtac1, Cryab, Cs, Cse1l, Csrp1, Ctbp1, Cul3, Cx3cl1, Cyfip2, Cyp46a1, Dbn1, Dclk1, Dclk2, Ddah1, Ddx1, Dhrs1, Diras1, Diras2, Dkc1, Dlat, Dlgap2, Dmd, Dpysl3, Dynll2, Edil3, Eif2b4, Bem, Endod1, Eno1, Epb41l1, Epb41l3, Erlin2, Exoc5, Ezr, Faah, Fam234b, Fbxl16, Fbxo41, Fkbp5, Fn3k, Gabrb2, Gabrg2, Galk2, Gap43, Gart, Gdpd1, GFAP, Glul, Gmppa, Gmpr, Gnao1, Gnaz, Gnb4, Gng2, Gng7, Golga7b, Gpd1l, Gpr37, Gria3, Gria4, Grin1, Gstp1, Hapln1, Hdac2, Hdlbp, Hpca, Hpcal4, Hspa4l, Hspb1, Hsph1, Igsf8, Inpp5f, Iqsec2, Itgb1, Itsn1, Kcnab2, Kcnk1, Kctd12, Kif3a, Ldha, Lin7a, Lmna, Lpar1, Lsamp, Ly6h, Lynx1, Map1a, Map1b, MAP2, Map6, Map7d1, Mapk3, Mapre3, Marcks, Mboat2, Mcts1, Me1, Mif, Mpdu1, Mpp6, Mtap, Myh10, Nae1, Nat8l, Ncald, Ncam1, Ncdn, Nckap1, Nckipsd, Nefl, Nme3, Nop56, Nrgn, Ntm, Ocrl, Ola1, Osbpl1a, Pacsin1, Pafah1b1, Pfkfb2, Pfkm, Pfkp, Pgbd5, Pgd, Pgm2l1, Phf24, Phyhip, Pik3r4, Pip4k2a, Pitpna, Pitpnc1, Plcb1, Plcl1, Plxna4, Por, Ppm1e, Ppp1r7, Ppp2r5e, Prdx1, Prex1, Prkar2b, Prkcb, Prkcd, Prpf19, Prps1, Prrt2, Prrt3, Ptdss2, Pten, Ptgr2, Ptpn5, Pygm, Rab3c, Rab6b, Rala, Rap1a, Rap1b, Rap2a, Rcc2, Rdh14, Reep2, Reep5, Rgs6, Rps18, Rps6, Rrbp1, Rtn1, Ryr2, S100a13, Scarb2, Scn1b, Scn2b, Scn4b, Sec31a, Sec61a1, Serinc5, Shisa4, Sipa1l1, Slc16a1, Slc17a6, Slc17a7, Slc27a4, Slc30a3, Slc4a10, Slc6a17, Slc7a8, Slc9a3r1, Smarca5, Snap25, Snrpd3, Snta1, Snx27, Spr, Ssr4, Stx1b, Stxbp5l, Sucla2, Sv2b, Sv2c, Syngr1, Syt1, Syt2, Syt3, Syt7, Them6, Thy1, Tmem63b, Tmx1, Tnr, Tppp, Tspan2, Tspan9, Ttyh2, Tuba8, Tubb2b, Tubb3, Uba2, Ube2n, Usp10, Usp14, Vamp1, Vamp2, Vcan, Vdac2, Vkorc1l1, Vps52, Vsnl1, Wfs1, Xpo6, Ybx1

C1qa, C1qb, C1qc, Ctsb, P2ry12, Sirpa

Acsl6, Aldh1l1, Aqp4, Atp1b2, Bcan, Dclk1, Entpd2, GFAP, Gja1, Gpr37l1, Gria2, Mfge8, Slc4a4, Slc6a1, Slc6a11, Slc7a10, Tlcd1, Trim9

Apod, Gpr37, Lpar1, Mbp, Mog, Plp1, Tspan2, Tubb4a

Common EV proteins

CD9, CD63, CD81, CD82

Anxa1, Anxa2, Anxa3, Anxa5, Anxa6, Anxa7, Anxa11

Rab1A, Rab1b, Rab2a, Rab2b, Rab3a, Rab3b, Rab3d, Rab4a, Rab4b, Rab5a, Rab5b, Rab5c, Rab6a, Rab6b, Rab7a, Rab8a, Rab8b, Rab10, Rab11b, Rab12, Rab14, Rab18, Rab21, Rab23, Rab35

Hsd17b12, Hsd17b4, Hsp90aa1, Hsp90ab1, Hspa12a, Hspa2, Hspa4, Hspa4l, Hspa5, Hspa8, Hspb1, Hspd1, Hsph1

EEA1, Vps4b, Vta1

Absence of non-EV proteins

Bcl-2, GM130, Nup, Cyc1

The complete MS proteomics data are available via PRIDE with identifier PXD031947