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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: A novel isolation method for spontaneously released extracellular vesicles from brain tissue and its implications for stress-driven brain pathology

Fig. 3

The release method enriches for EV-associated proteins. a Volcano plot of the detected proteins of the GO category “extracellular exosome”. b Venn diagram of the detected proteins between release and standard method. c Cellular component analysis of EVs proteome exhibiting higher enrichment of categories related to small EVs (e.g., exosomes) in the release method (based on ConsensusPathDB). d Tissue derivation of EV proteins of the release method (based on DAVID knowledgebase), showing enrichment in brain- and hippocampus-specific proteins. e Representative immunoblots of mouse brain EVs from the standard and release methods, showing the presence of EV-related proteins (e.g., CD81, flotillin) and endosomal proteins, (e.g., EEA-1), but not the mitochondrial protein cytochrome c (N = 3 per condition)

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Cell Communication and Signaling

ISSN: 1478-811X