Fig. 1
Binding of CrkL to full-length C3G. A Schematic representation of the domain structure of C3G. B ITC analysis of CrkL binding to C3G. Upper panel shows thermograms of the dilutions of CrkL in buffer (black line) and of the titration of 4.8 µM C3G with CrkL (orange line). The middle panel shows the binding isotherms of three independent titrations as indicated; lines are the theoretical binding curves obtained by the global fit. Residuals from the fitted model are shown in the lower panel. C Sedimentation coefficient distributions of C3G alone and in the presence of increasing concentrations of CrkL as indicated. D Isotherms of the sfast obtained from the integration of the fastest c(s) peaks in (C), and sw determined from the integration of the entire c(s) distribution. E Signal amplitudes cfast and cslow determined by the integration of the c(s) peaks. Dashed lines in (D)-(E) represent the best fit using the three-sites binding model based on the Gilbert-Jenkins theory