Fig. 9

CD38 shRNA or overexpressed plasmid was successfully transfected into MLE-12 cells and Daph up-regulated CD38 expression. a The molecular docking diagram of Daph with CD38 molecule. b The mRNA expression of CD38 after Daph stimulating MLE-12 cells were measured by RT-qPCR. c The protein expression of CD38 in MLE-12 cells were detected by Western blot analysis, and relative expression of CD38 to GAPDH was analyzed by Image J software. d The protein expression of CD38 in MLE-12 cells after CD38 shRNA transfection were detected by Western blot analysis, and relative expression of CD38 to GAPDH was analyzed by Image J software. e–f MLE-12 cells were transfected with CD38 shRNA or overexpression plasmid for 24 h and followed by LPS and Daph treatment as indicated, the protein expression of CD38 was detected by Western blot, and relative expression of CD38 to GAPDH were analyzed by Image J software. All results were expressed as the means ± SD of three independent experiments. *P < 0.05, **P < 0.01 versus 0 μg/ml Daph or the control group; $$P < 0.01, ##P < 0.01 versus LPS group; &&P < 0.01 versus LPS + Daph group