Fig. 6

LPS could stimulate inflammatory cytokines expression in MLE-12 cells. a-b The viability of MLE-12 cells was analyzed by the CCK-8 assay after LPS or Daph challenge for 24 h. c-f The mRNA expression of IL-1β, IL-18, IL-6 and iNOS in cells were measured by RT-qPCR. g The protein expression of IL-1β, IL-18, IL-6 and iNOS were measured by Western blot analysis, and the relative expression of previous protein were quantified by Image J software and GAPDH was acted as an internal control. All results were expressed as the means ± SD of three independent experiments. *P < 0.05, **P < 0.01 versus 0 μg/ml LPS or 0 μg/ml Daph