Fig. 4

Effects of Daph on TLR4-NF-κB/MAPK signaling pathway activation in LPS-induced lung injury. a-b The protein expression of TLR4, MyD88, p-NF-κB p65, NF-κB p65, p-ERK1/2, ERK1/2, p-JNK, JNK, p-p38 and p38 in lung tissue were detected by Western blot analysis, and relative expression of previous protein were quantified by Image J software and GAPDH was acted as an internal control. c The distribution of NF-κB p65, ERK1/2 and JNK in lung tissue were detected by Immunohistochemistry staining. d The calculated NF-κB p65, ERK1/2, JNK-positive cells (brown area) in lung tissue for all groups. All results were expressed as the means ± SD of three independent experiments. **P < 0.01 versus the control group; ##P < 0.01 versus LPS group