Fig. 3

Daph treatment alleviated pulmonary inflammation and reduced mortality in LPS-induced lung injury. a-f The mRNA expression of IL-1β, IL-18, IL-6, iNOS, MCP-1 and CCR2 in lung tissue were measured by RT-qPCR. g The protein expression of IL-6, iNOS, MCP-1 and CCR2 were detected by Western blot analysis, and relative expression of previous protein were quantified by Image J software and GAPDH was acted as an internal control. h The survival rates of the sepsis mice were observed after Daph treatment, WT + PBS, WT + DMSO and WT + Daph were used as the control group among them, so these three lines overlapped together. i-j The pathological changes in lung tissue were compared by H&E staining, and the lung injury scores were evaluated. The black arrows indicate inflammatory cell infiltrations, the green arrows indicate thickened of alveolar walls, the red arrows indicate intrapulmonary hemorrhage and the blue cycles indicate formation of edema fluid. All results were expressed as the means ± SD of three independent experiments. **P < 0.01 versus the control group; #P < 0.05, ##P < 0.01 versus LPS group