Fig. 12

CD38 Overexpression reduced inflammation in MLE-12 cells induced by LPS. a-d The mRNA expression of inflammatory cytokines IL-1β, IL-18, IL-6 and iNOS in cells were measured by RT-qPCR. e–g The protein expression of IL-6, iNOS, TLR4, MyD88, NF-κB p65, p-NF-κB p65 p-ERK1/2, ERK1/2, p-JNK, JNK, p-p38, p38 in cells were measured by Western blot analysis, and relative expression of previous protein were quantified by Image J software and GAPDH was acted as an internal control. All results were expressed as the means ± SD of three independent experiments. **P < 0.01 versus the control group; $P < 0.05, $$P < 0.01, ##P < 0.01 versus LPS group; &P < 0.05, &&P < 0.01 versus LPS + Daph group