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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Endothelial cell-derived MMP19 promotes pulmonary fibrosis by inducing E(nd)MT and monocyte infiltration

Fig. 3

MMP19 promotes E(nd)MT, migration and permeability of HPMECs. A Immunofluorescence analysis for CD31 (red) and α-SMA (green) in HPMECs treated as indicated. B Immunofluorescence analysis for VE-Cadherin (red) and α-SMA (green) in HPMECs treated as indicated. C qRT-PCR was performed for markers of E(nd)MT in HPMECs 6 h after transient transfection with full length MMP19 and MMP19E213A cDNA (CDH2 = N-cadherin; VIM = vimentin). D Immunoblot for markers of E(nd)MT in HPMECs 24 h after transient transfection with MMP19WT and MMP19E213A cDNA. E Immunoblot for markers of E(nd)MT in HPMECs 24 h after rhMMP19 treatment. F Assay of permeability of HPMECs monolayers following transfection of MMP19WT, and MMP19E213A cDNA to FITC-albumin. G Scratch assay was performed on HPMECs following transfection of MMP19WT and MMP19E213A cDNA. H Quantification of the cell migration distance. I Transwell assay was used to measure HPMECs migration. J Quantification of the number of cells that migrated to lower chambers

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Cell Communication and Signaling

ISSN: 1478-811X