Fig. 6

p-STAT6 promotes the expression of c-Myc as its transcription factor and then accelerates the proliferation of Ph+ ALL cells. After treatment with different concentrations of AS1517499 for 48 h, a the expression of c-Myc, p27, p21, Cdk1, Cdc25c, and PCNA in BP190 and SUP-B15 cells was measured by western blot assay; b the mRNA of c-Myc in BP190 and SUP-B15 cells was measured by qRT-PCR assay. c Co-localization of p-STAT6 and c-Myc was detected by IF assay, scale bar, 10 μm. d Schematic diagram of the c-Myc promoter region and P1 or P2 gene fragments. e PGL3-NL, P1 or P2, and STAT6 plasmids were co-transfected into 293 T cells for 48 h, the luciferase activity was detected by the dual-luciferase assay. f Schematic diagram of primers binding to the sequence of c-Myc promoter region. g The enrichment of c-Myc gene fragments was analyzed by ChIP-qPCR assay. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. ns indicates no significant differences