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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Jak2/STAT6/c-Myc pathway is vital to the pathogenicity of Philadelphia-positive acute lymphoblastic leukemia caused by P190BCR-ABL

Fig. 2

STAT6 is activated by BCR-ABL in P190 cell Lines, and P190 cell lines is more sensitive to the inhibition of STAT6 activation than P210 cell lines. a The mRNA levels of STAT6 in Ba/F3, BP210, BP190, K562, and SUP-B15 cells were analyzed by qRT-PCR assay. b The protein levels of STAT6 and p-STAT6 in Ba/F3, BP210, BP190, K562, and SUP-B15 cells were analyzed by western blot assay. c The expression of p-STAT6 was detected by IF assay. d The expression of BCR-ABL, STAT6, and p-STAT6 in BP190 and SUP-B15 cells treated with IM for 48 h was detected by western blot assay. e The IC50 values of AS1517499 treated BP210, BP190, K562 and SUP-B15 cells for 48 h were measured by CCK-8 assay. SUP-B15 and BP190 cells treated with different concentrations of AS1517499 for 48 h, f the expression of p-STAT6 and STAT6 was measured by western blot assay, g and the expression of STAT6 in nucleus and cytoplasm was measured by IF assay. *p < 0.05. ns indicates no significant differences

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Cell Communication and Signaling

ISSN: 1478-811X