Fig. 8

Demyelination analysis of spinal axons in SCI mice after treatment. A Luxol fast blue staining of axial sections of the epicenter from the lesion level at 28 dpi in control, SCI injury, A1 astrocyte-transplanted, and A2 astrocyte-transplanted groups. Scale bar = 200 μm at low magnification; scale bar = 50 μm at high magnification. B Quantification of Luxol fast blue staining in the injured area relative to the control group. C Electron micrographs of transverse sections of the spinal cord 28 days after spinal contusion injury in control, SCI injury, A1 astrocyte-transplanted, and A2 astrocyte-transplanted groups. Scale bar = 5 μm. D Percentage and E number of myelinated axons. F G-ratio in the four groups. Error bars show means ± SD (n = 6 in each group). **p < 0.01 compared to the injury group