Fig. 7

Identification of A1 and A2 astrocytes within the spinal cord 28 days post injury after cell transplantation. A Immunofluorescence staining for C3 (A1 astrocyte marker: red) and S100A10 (A2 astrocyte marker: green) in the injury site of the spinal cord at 28 dpi after SCI and astrocytes transplantation treatment. Scale bar = 500 μm at low magnification. Scale bar = 100 μm at high magnification. B Quantification of the S100A10-positive area of the injured spinal cord at 28 dpi. C Quantification of the C3-positive area of the injured spinal cord at 28 dpi. D–F RT-PCR analysis of A1-specific genes: Fbln5, Serping1, and Srgn relative expression and G–I A2-specific genes: Clcf1, Tgm1, and Emp1 relative expression in the spinal cord at the injured level from control, injury, A1 and A2 groups. All mRNA expression was normalized to GAPDH. Error bars showed means ± SD (n = 6 in each group). **p < 0.01, ^##p < 0.01 compared to injury group