Fig. 4

Assessment of locomotor recovery after cell transplantation. A Treatment schedule of astrocyte transplantation for the mouse model of spinal cord injury. B A footprint analysis performed at 28 dpi in control, spinal cord injury, A1 astrocyte-transplanted, and A2 astrocyte-transplanted groups. Scale bar = 1 cm. Quantification of the footprint analysis after SCI at 28 dpi by stride length (C) and width (D). E Representative images of the swimming tests in the four groups. F Quantification of (E) by the swimming score. G Time-dependent Basso mouse scale in SCI mice after hydrogel alone, A1 astrocytes wrapped in hydrogel, or A2 astrocytes wrapped in hydrogel at 1, 3, 7, 14, 21, and 28 dpi. H Time-dependent hindlimb reflex scoring in SCI mice after hydrogel alone, A1 astrocytes wrapped in hydrogel, or A2 astrocytes wrapped in hydrogel at 0, 1, 3, 7, 14, 21, and 28 dpi. Error bars show means ± SD (n = 6 in each group). *p < 0.05, **p < 0.01 compared to the injury group