Fig. 3

Effect of differentiated astrocyte on neuronal apoptosis and complexity. A Identification of neurons by MAP2 and NeuN immunofluorescence staining. Scale bar = 100 μm. B Representative images of flow cytometry assay results labeled with PI and annexin-V-FITC in primary neurons co-cultured with differentiated astrocytes after GLU-induced excitotoxicity. C Quantification of (B): percentage of apoptotic neurons in different groups. D Western blot analysis of apoptosis-related proteins in primary neurons. E Quantification of (D). F Representative images of MAP2 immunostaining for primary neurons in each experimental group. G Quantification of intersections by Sholl analysis. *p < 0.05, **p < 0.01