Fig. 2

Analysis of A1 and A2 markers of astrocytes after LPS-activated DMEM or IL-4-activated DMEM culture. A Western blot analysis of C3 (as an A1 astrocyte marker) and S100A10 (as an A2 astrocyte marker) after astrocytes were induced to differentiate. B, C Quantification of (A). D Immunofluorescence staining for C3 and S100A10 in control, LPS-activated DMEM, and IL-4-activated DMEM group on day 3. Scale bar = 100 μm. E Quantification of C3-positive cells. F Quantification of S100A10-positive cells. Error bars show mean ± SD (n = 3 in each group). **p < 0.01, ^##p < 0.01 compared to control group