Fig. 6
Cth silencing or overexpression in macrophages leads to downstream changes in ERK1/2 and an efferocytosis phenotype in vitro. A Bar graphs showing no effect on cell status after cth silencing or overexpression in macrophages. Top: The CCK8 results showing the changes in viability in each group of cells. CCK8, cell counting kit-8. Bottom: Flow cytometry showing the mortality of each group of cells. B, C Bar graphs showing changes in the gene and protein levels of the ERK1/2 pathway and efferocytosis biomarkers (AXL, CD36, UCP2) after alterations in Cth. Control: Mouse peritoneal macrophages; ControlOE: Control overexpression of mouse peritoneal macrophages; CthOE: Cth overexpression in mouse peritoneal macrophages; Controlsi: Cth silencing in mouse peritoneal macrophages; Cthsi: Control silencing in mouse peritoneal macrophages. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001, one-way ANOVA with Tukey’s multiple comparisons test