Fig. 5

Cth induces macrophage efferocytosis in an ERK1/2-dependent manner after I/R. A Representative immunofluorescence images showing the colocalization of Cth, ERK1/2 and macrophages following alterations in Cth and ERK1/2. Rabbit anti-CTH/FITC labels Cth (green); rabbit anti-phospho-ERK1/2/PE labels ERK1/2 (cyan); APC anti-F4/80 labels macrophages (red). B, C The levels of Cth, ERK1/2 and the biomarkers of efferocytosis (AXL, CD36, UCP2) were measured using q-PCR and western blotting. n = 6/group. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001, one-way ANOVA with Tukey’s multiple comparisons test. The subgroups and interventions were identical to those in Fig. 4