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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Cystathionine gamma-lyase (Cth) induces efferocytosis in macrophages via ERK1/2 to modulate intestinal barrier repair

Fig. 2

In vivo, efferocytosis was present in the damaged intestine, and its level gradually increases with prolonged I/R time. A Schematic diagram of the in vivo efferocytosis assay. B Changes in the efferocytosis index (proportion of macrophages engulfing apoptotic endothelial cells to total macrophages). C Representative immunofluorescence images showing macrophage phagocytosis of apoptotic endothelial cells in the intestine of live animal models. CellTracker™ Deep Red Dye labels apoptotic IEC-6 cells (red); F4/80 PE antibody labels macrophages. D Gene expression of efferocytosis biomarkers (AXL, CD36, UCP2). Sham: sham-operated group; 12 h: 12-h post-I/R group; 24 h: 24-h post-I/R group; 36 h: 36-h post-I/R group; 48 h: 48-h post-I/R group. n = 6/group. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001, one-way ANOVA with Tukey’s multiple comparisons test

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Cell Communication and Signaling

ISSN: 1478-811X