Fig. 6

Ectopic expression of SEMA7A promotes NF-κB p105 procession and activates the NF-κB p50/p65 pathway. a and b Relative Sema7a expression in HCC cell lines (HepG2, Huh7, PLC5, and Hep3B) compared with an immortalized normal human hepatic cell line (L02). c Sema7a overexpression efficiency and relative NF-κB1, TNFα and IL1β mRNA levels in transfected HepG2 cell lines (n = 3). d and e Western blotting analysis of the relative levels of Sema7a, integrin β1, NF-κB p105, NF-κB p50, p- NF-κB p65/NF-κB p65, p-IκB/IκB, TNFα and IL1β protein expression in transfected HepG2 cell lines following integrin β1 silencing (n = 3). Phosphorylation levels were measured by the phosphor/total protein ratio. The qPCR data were analysed by the independent-samples Student’s t test and differences among the groups were determined by one-way ANOVA with Tukey’s post hoc tests or by Kruskal‒Wallis test with Dunn’s post hoc test analysis. *Means p < 0.05