Fig. 5

SEMA7A is elevated in hepatocellular carcinoma. a The mRNA level of SEMA7A in liver hepatocellular carcinoma from TCGA. b and c The mRNA levels of SEMA7A and integrin β1 in four tumour grades. d Characteristic images of liver tissue from wild-type and hepatocellular carcinoma mice. e Representative images of H&E staining in wild-type and hepatocellular carcinoma. f, g Western blotting analysis of the relative levels of Sema7a, integrin β1, NF-κB p105, p- NF-κB p50/NF-κB p50, p- NF-κB p65/NF-κB p65, p-IκB/IκB, TNFα and IL1β protein expression in the livers of wild-type (n = 5) and hepatocellular carcinoma mice (n = 5). h The relative levels of mRNA transcripts of the genes for SEMA7A in wild-type (n = 5) and hepatocellular carcinoma mice (n = 5). i Coimmunoprecipitation analysis of protein interactions among integrin β1 and NF-κB p105 in liver tissues from wild-type and hepatocellular carcinoma mice. The data were analysed by the independent-samples Student’s t test. *Means p < 0.05 versus wild-type mice