Fig. 3

Integrin β1 binds to NF-κB p105 and promotes the NF-κB p50/65 pathway. a The protein‒protein interaction network of the Sema7a, integrin β1 and NF-κB protein families was predicted by the Retrieval of Interacting Genes Database. b Coimmunoprecipitation analysis of protein interactions among integrin β1 and NF-κB p105 in liver tissues from 8-week-old male wild-type and Sema7a.^R145W homozygous mice. The higher band is NF-κB p105 (105 kDa) and the lower band is NF-κB p55 (55 kDa). c Schematic showing the primary structure and truncation of NF-κB p105. d and e Coimmunoprecipitation analysis of His-tagged NF-κB p105 and Flag-tagged integrin β1 in HEK293 cells. f, g Co-immunoprecipitation analysis of His-tagged NF-κB p55 and Flag-tagged integrin β1 in HEK293 cells. h Coimmunoprecipitation analysis of the protein interaction between Sema7a and NF-κB p105 in primary mouse hepatocytes after integrin β1 silencing (n = 3). i, j Western blotting analysis of the relative levels of integrin β1, NF-κB p105, p- NF-κB p50/NF-κB p50, p- NF-κB p65/NF-κB p65, p-IκB/IκB, TNFα and IL1β protein expression in primary hepatocytes following integrin β1 silencing (n = 3). Phosphorylation levels were measured by the phosphor/total protein ratio. The difference among the groups was determined by one-way ANOVA with Tukey’s post hoc tests or by Kruskal‒Wallis test with Dunn’s post hoc test analysis. *Means p < 0.05