Fig. 2

The NF-κB pathway is activated in Sema7a^R145W mouse liver and primary mouse hepatocytes. a–c Western blotting analysis of the relative protein levels of NF-κB p105, p- NF-κB p50/NF-κB p50, p- NF-κB p65/NF-κB p65 and p-IκB/IκB in the livers of wild-type (n = 4) and Sema7a^R145W homozygous male mice (n = 5) and b, d in primary hepatocytes from wild-type (n = 3) and Sema7a^R145W homozygous (n = 3) male mice. Phosphorylation levels were measured by the phosphor/total protein ratio. e Representative IF staining showing p-NF-κB p65 Ser529 (red) and DAPI (blue). Scale bars: 50 μm in liver sections from wild-type and Sema7a^R145W homozygous male mice. f The relative levels of mRNA transcripts of the genes for NF-κB p105 in wild-type (n = 4) and Sema7a^R145W homozygous male mice (n = 5) and g in primary hepatocytes from wild-type (n = 3) and Sema7a^R145W homozygous (n = 3) male mice. The data were analysed by the independent-samples Student’s t test. * means p < 0.05 versus wild-type mice