Fig. 8
From: NEDD4 ameliorates myocardial reperfusion injury by preventing macrophages pyroptosis
NEDD4 regulates SF3A2 ubiquitination indirectly inhibiting NLRP3 inflammasome activation. A, B HEK293T cells were transfected with Myc-SF3A2 and HA-UB, together with the empty control vector or Flag-NEDD4 expression vector. Cell lysates were immunoprecipitated with anti-RIP1 antibody after treatment with or without LPS and Nig stimulation. C CMECs transfected with GFP- and RFC-NLRP3 were fixed and incubated with a secondary antibody conjugated to Alexa Fluor 594 and Alexa Fluor 488. Colocalization between NEDD4 and NLRP3 was examined by fluorescence microscope