Fig. 7
From: NEDD4 ameliorates myocardial reperfusion injury by preventing macrophages pyroptosis
SF3A2 positively regulates NLRP3 activation and pyroptosis. A, B Immunoblot analysis and quantification by densitometry of SF3A2 and NLRP3 protein in both H9c2 and CMECs cultured under normoxia or subjected to hypoxia/reoxygenation (H/R) (2/2 h). Data are expressed as mean ± SD (n = 3). *P <0.05 vs. normoxia. C–G Immunoblot analysis and quantification by densitometry of NLRP3, Caspase-1 (p20), GSDMD and C-GSDMD protein in lysates from THP-1 cells transfected with si-SF3A2, primed with LPS 4 h, and followed by stimulation with Nig for 1 h. Data are expressed as mean ± SD (n = 3). *P<0.05 vs. si-control group. H Lysates of THP-1 cells treated with LPS for 4 h, and followed by stimulation with Nig for 1 h were immunoprecipitated with anti-SF3A2 antibody. I Immunofluorescence staining for SF3A2 and NLRP3 colocalization in CMECs after conditioned medium stimulation from LPS/Nig-activated or control THP-1 cells. J Immunofluorescence staining for SF3A2 and NLRP3 colocalization in THP-1 cells with or without stimulation with LPS and Nig