Fig. 4
From: NEDD4 ameliorates myocardial reperfusion injury by preventing macrophages pyroptosis
NEDD4 negatively regulates NLRP3 activation and pyroptosis. A ELISA of GSDMD, IL-1β and TNF-α in supernatants from NEDD4+/+ or NEDD4−/− mouse treated with LPS for 6 h, and followed by stimulation with ATP, Nig. or poly (dA:dT) for 2 h. Data are expressed as mean ± SD (n = 5). *P < 0.05 vs. NEDD4+/+ group. B ELISA of GSDMD, IL-1β and TNF-α in supernatants from mouse peritoneal macrophages silenced of NEDD4, primed with LPS for 6 h, and followed by stimulation with ATP, Nig. and poly (dA:dT) for 2 h. Data are expressed as mean ± SD (n = 5). *P < 0.05 vs. ctrl siRNA group. C–G Immunoblot analysis and quantification by densitometry of NLRP3, Caspase-1 (p20), GSDMD and C-GSDMD protein in lysates from THP-1 cells transfected with si-NEDD4, primed with LPS 4 h, and followed by stimulation with Nig for 1 h. Data are expressed as mean ± SD (n = 3). **P < 0.05 vs. ctrl siRNA group. H Immunofluorescence staining for NLRP3 in CMECs transfected with or without si-NEDD4. I-M Immunoblot analysis and quantification by densitometry of NLRP3, Caspase-1 (p20), GSDMD and C-GSDMD protein in lysates from mouse peritoneal macrophages silenced of NEDD4, primed with LPS 4 h, and followed by stimulation with Nig for 1 h. Data are expressed as mean ± SD (n = 3). *P < 0.05 vs. NEDD4+/+ group