Fig. 3
From: NEDD4 ameliorates myocardial reperfusion injury by preventing macrophages pyroptosis
Effect of NLRP3 inflammasome activation in macrophages on CMECs. A–E Immunoblot analysis and quantification by densitometry of NLRP3, Caspase-1 (p20), GSDMD and C-GSDMD protein in lysates (n=3). F–G ELISA analysis supernatants of IL-1β and GSDMD in THP-1 cells treated with LPS (1μg/ml), and followed by stimulation with10 μM nigericin (n=3). H–L Immunoblot analysis and quantification by densitometry of NLRP3, Caspase-1 (p20), GSDMD and C-GSDMD protein in lysates from THP-1 cells transfected with si-NLRP3 (n=3). M ELISA analysis supernatants of IL-1β and GSDMD in THP-1 cells treated with si-NLRP3. N–O Immunofluorescence staining for GSDMD and caspase-3 in CMECs at 12 h after conditioned medium stimulation from LPS/Nig-activated or control THP-1 cells with or without transfected with si-NLRP3 (n=5). Scar bar=20μm