Fig. 7

Mechanotransduction contributes to ESC proliferation during uterine regeneration in a Rap1a/ARHGAP35/RhoA/F-actin YAP-dependent manner. a, b Immunofluorescence images (a) and quantification of Ki67-positive cells (b) in ESC cultures transfected with si NC, si YAP, si Rap1a or pcDNA3.1(+) Rap1a at 40 kPa/1 kPa hydrogels. Scale bars, 200 μm. c CCK-8 kits were used to assess the proliferation of ESCs transfected with siYAP or pcDNA3.1(+) YAP at 0, 12, 24, 48 and 72 h. d–h Uterine tissues were collected from inflammatory-infected and postpartum mice treated with C3, Y27632 and Cyto. B on Day 20, Day 5, Day 22 or Day 10. d Percentage of sections encompassing individual morphological stages at Day 22 or Day 10. e, f Confocal immunofluorescence images (e) and quantification of BrdU+ positive cells (f) in uterine sections. Scale bar: 100 μm. g, h Confocal immunofluorescence images (g) and quantification of the nuclear and cytoplasmic subcellular localization of YAP (h). Scale bar: 100 μm, 10 μm (bottom). Data are means ± S.E.M. of at least three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001