Fig. 4

YAP responds to mechanical signaling via focal adhesion in ESCs. a, b Confocal immunofluorescence images (a) and quantification of nuclear and cytoplasmic subcellular localization (b) of YAP in ESCs plated on hydrogels with different rigidities. Scale bars, 20 μm. c Western blotting for YAP in nuclear and cytoplasmic protein fractions from ESCs plated on 1 kPa or 40 kPa fibronectin-coated hydrogels for 24 h. d Representative immunofluorescence images of vinculin in ESCs cultured on 1 kPa and 40 kPa hydrogels, n = 2. Scale bars, 10 μm. e, f Immunofluorescence images of YAP and vinculin (e) and quantification of YAP+ positive cells (f) in ESCs. Scale bar: 10 μm. g Lysates of ESCs transfected with si vinculin were analyzed for the presence of the indicated protein. Data are means ± S.E.M. of at least three independent experiments. ***P < 0.001