Fig. 2

Changes in biomechanical cues during endometrial regeneration. a Gene set enrichment analysis (GSEA) of RNA-seq data for menstrual and proliferative cells revealed enrichment of cell‒cell adhesion gene signatures, ECM structural signaling-related genes, and cell matrix adhesion in proliferative cells. The normalized enrichment scores (NES) were 2.51, 1.95 and 2.10, respectively. The false discovery rates (FDR) were 0.000, 0.045 and 0.003, respectively. P < 0.001. b, c Immunofluorescence staining of α-PKC and E-cad (b) and F-actin labeled by phalloidin and ZO-1 (c) in sections of mouse uterus. Scale bar: 100 μm. d Uterine explants obtained at different repair phases at the beginning and 24 h later (left) and changes in uterine explant area over a period of 24 h (right). Scale bars, 1 mm. e Lysates of uterine tissue obtained at different repair phases were analyzed for the presence of the indicated proteins. Data are means ± S.E.M. of at least three independent experiments. *P < 0.05; ***P < 0.001