Fig. 1

Endometrial injury and recovery in inflammatory-infected mouse uterus. Uterine tissues and blood were collected at 1, 2, 4, 6, and 8 d after injection of LPS. a White blood cell (WBC) content was analyzed by routine blood examination. n = 10–12 mice per group. b Representative histopathological images of paraffin-embedded mouse uterine sections. Scale bar: 200 μm (top); 20 μm (bottom). c–f Representative images (left panels) and quantification (right panels) of caspase 3 (c, d) and BrdU (e, f) expression in the indicated tissues from mice with inflammatory damage and in control mice. Scale bars, 200 μm. g, h Immunostaining of CD11b and CD45 (g) and quantification (h) of their staining in uterine tissue sections from mice with inflammatory damage. Scale bar: 20 μm. i. mRNA levels of IL-6, IL-1β, TNF-α and IL-10 in the endometrium were measured by RT‒qPCR. Data are means ± S.E.M. of at least three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001