Fig. 7
From: Klf4-Sirt3/Pparα-Lcad pathway contributes to high phosphate-induced lipid degradation
Phosphorus incubation induced lipid degradation by activating KLF4 in the IECs of yellow catfish. A Semi-quantitative of si-klf4 mRNA expression. B Relative mRNA expression of si-klf4. C, D Western blot analysis and quantification analysis of siklf4-358/797/1164. E TG content. F Presence of LDs stained with Bodipy 493/503 was demonstrated by flow cytometry. G Lipid content was quantified by flow cytometric analysis of FL1 (green) mean fluorescence intensity with BODIPY 493/503 staining. H Representative confocal microscopy images of primary IECs in yellow catfish with BODIPY 493/503 staining. Bars represent 25 μm. I, J Specific enzymes in the pathway map showing mitochondria fatty acid oxidation. The mRNA levels of fatty acid β-oxidation related genes are indicated by color coding: the mRNAs with increased levels are marked in red, and with decreased or unaltered levels are marked in black. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 (One-way ANOVA with the Bonferroni post hoc test)