Fig. 6
From: Klf4-Sirt3/Pparα-Lcad pathway contributes to high phosphate-induced lipid degradation
Pi incubation activated SIRT3-LCAD and PPARα-LCAD pathway in the IECs. A The mRNA levels of lcad. B, C Western blot analysis and quantification analysis of LCAD protein levels. D Interaction of SIRT3 with LCAD under Pi incubation. E Interaction of SIRT3 with LCAD, Flag-tag LCAD and HA-tag SIRT3 were transfected into 293T cells, and the interaction between SIRT3 and LCAD was determined with IP and western blot. F PPARα response elements (PPRE) located at − 1422 bp to − 1397 bp of lcad promoter. G Relative luciferase activity of lcad promoter. H EMSA of putative LCAD binding sequences (PPRE). *P < 0.05, compared with the control