Fig. 5
From: Klf4-Sirt3/Pparα-Lcad pathway contributes to high phosphate-induced lipid degradation
Phosphorus incubation activated KLF4-SIRT3 and KLF4-PPARα pathway in the intestinal epithelial cells (IECs) of yellow catfish. A The mRNA levels of klf4, sirt3, pparα. B, C Western blot analysis and quantification analysis of KLF4, SIRT3, PPARα protein levels. D Relative luciferase activity of sirt3 promoter. E Relative luciferase activity of pparα promoter. F EMSA of putative SIRT3 binding sequences (ERE). G EMSA of putative PPARα binding sequences (KRE). All data were expressed as mean ± S.E.M. (n = 3). P value was calculated by Student’s t tests. *P < 0.05, compared with control. IP, immunoprecipitation; NS, non-significance