Fig. 4
From: Klf4-Sirt3/Pparα-Lcad pathway contributes to high phosphate-induced lipid degradation
Phosphorus incubation induced lipolysis and influenced mitochondrial homeostasis in the intestinal epithelial cells (IECs) of yellow catfish. A Pi content. B TG content. C CPT1 activities. D NEFA content. E Representative confocal microscopy images of primary IECs in yellow catfish with BODIPY 493/503 staining. Scale bar, 25 μm. F Presence of LDs stained with BODIPY 493/503 demonstrated by flow cytometry. G Lipid content quantified by flow cytometric analysis of FL1 (bule) mean fluorescence intensity with Bodipy 493/503 staining. H ATP content. I NAD+, NADH and total NAD contents. J NAD+/NADH ratio. All data were expressed as mean ± S.E.M. (n = 3). *P < 0.05, compared with the control