Fig. 5

Functional changes in HMEC-1 cells caused by NMU. a Markers of TECs, IGFBP-7 and vimentin, in HMEC-1 cells treated with NMU-9 (VEGF-A as a positive control) were analysed using immunoblotting. The image shows a representative result. The bands were quantified using densitometry. The intensity of the bands was normalized to the loading control (GAPDH). The results are presented as the means with min-to-max ranges (ordinary one-way ANOVA, Dunnett's multiple comparisons test: *p ≤ 0.05; n ≥ 3). b Migration and c microtube formation abilities of HMEC-1 cells treated with NMU-9 (left panel) or CM from HT29 cells secreting NMU (right panel) (VEGF-A served as a positive control). b The extent of wound closure at 24 h during the experiment was compared with the initial measurement. The results are presented as the means with min-to-max ranges (RM one-way ANOVA, Dunnett’s multiple comparisons test: *p ≤ 0.05; n ≥ 4). The images show representative results. c Number of junctions and total length of segments formed by HMEC-1 cells treated with NMU-9 or CM containing NMU were compared with untreated cells or incubated with CM from pcDNA clones. The results are presented as the means with min-to-max ranges (one sample t test: *p ≤ 0.05; ***p ≤ 0.001; n ≥ 4). The images show representative results (scale bar = 100 µm)