Fig. 3

NMUR1 expression and activity in macrophages and endothelial cells. a NMUR1 expression in THP-1 cells and various phenotypes of TDMs (left graph), human PBMs and various phenotypes of MDMs (middle graph) and HMEC-1 cells (right graph). The results are presented as the means with SD (ordinary one-way ANOVA test, Dunnett's multiple comparisons test, *p ≤ 0.05; **p ≤ 0.01, n ≥ 5). b NMUR1 protein visualized using confocal microscopy (scale bar = 20 µm). c ERK1/2 activation after NMU-9 (250 nM) treatment of TDM M0 (left graph), MDM M0 (middle graph) and HMEC-1 (right graph) cells as analysed using immunoblotting. The image shows a representative result. The bands were quantified using densitometry. The intensity of the pERK1/2 bands was normalized to the respective ERK1/2 and α-tubulin bands. The results are presented as the means with min-to-max ranges (one sample t test, *p ≤ 0.05; n ≥ 3)