Fig. 1
From: Neuromedin U secreted by colorectal cancer cells promotes a tumour-supporting microenvironment
Human CRC tissues—IHC and TCGA data analysis. a Tissue samples (grade 1) collected on tissue microarrays stained with anti-NMU antibodies (IHC) and analysed under the microscope (zoom 20x, field 0.3 mm2). b Percent of all analysed cores with different levels of NMU staining (Fisher’s exact test). c Percent of NMU-positive epithelial cells in the analysed CRC tissues, grade 1 (Wilcoxon matched-pairs signed rank test). NAT—normal adjacent tissue. d Changes in NMU and NMURs expression in CRC tissues compared to normal samples based on the expression levels extracted from TCGA RNA sequencing dataset (published here [14]). e Ordered patient data with survival data were divided into NMUR low (n = 260) and NMUR high (n = 260) expression groups according to the median values, and survival rates were compared between the groups by performing a Kaplan–Meier analysis (log-rank (Mantel–Cox) test, χ2 = 6.559, df = 1, p = 0.0104). NMUR1 expression was extracted from TCGA RNA sequencing dataset and compared among CRC tumours with different f MSI statuses (Kruskal–Wallis test, p < 0.0001, Dunn’s multiple comparisons test, ***p = 0.0008; ****p < 0.0001), g M stages (Mann–Whitney test, two-tailed, p = 0.4419), h N stages (Mann–Whitney test, two-tailed, p = 0.0079), and i VEFGC expression between groups with NMUR1 low and NMUR1 high levels according to the median values (Mann–Whitney test, two-tailed, ****p < 0.0001). The boxes represent the interquartile ranges, the horizontal lines in the boxes represent the medians, and the whiskers represent the minimum and maximum values