Fig. 5

LncRNA-TBP interacts with TBP to regulate the transcriptional activity of TBP-target genes. A LncRNA-TBP interacts with TBP protein were determined by RNA Immunoprecipitation (RIP). B and C The interaction of full-length and truncated LncRNA-TBP (base pairs 1-417, 302-670 and 636-1057) (B) with TBP protein was determined by RNA pulldown (C). D ChIP-qPCR results showing that TBP enrichment at the KLF4, GPI, TNNI2, and CDLN1A promoters in CPMs (n = 3). E ChIP-qPCR results showing that LncRNA-TBP overexpression significantly increased TBP enrichment at the KLF4, GPI, TNNI2, and CDLN1A promoters in CPMs (n = 3). F–I The luciferase activities of the reporter show that the relative promoter activity of KLF4 (F), GPI (G), TNNI2 (H), and CDKN1A (I) significantly changed with LncRNA-TBP overexpression in CPMs (n = 6). J–K The relative mRNA (n = 4) (J) and protein (n = 3) (K) expression levels of KLF4, GPI, TNNI2, and CDKN1A after LncRNA-TBP overexpression. In panels A, C, and K, the numbers shown below the bands were folds of band intensities relative to control. Band intensities were quantified by ImageJ and normalized to β-Tubulin. Data are expressed as a fold-change relative to the control. Results are presented as mean ± SEM. In panels D–J, the statistical significance of differences between means was assessed using an independent sample t-test. (*P < 0.05; **P < 0.01; N.S., no significant difference)