Fig. 4

LncRNA-TBP activates slow-twitch muscle phenotype and induce muscle hypertrophy. A Relative enzymes activity of lactic dehydrogenase (LDH) and succinate dehydrogenase (SDH) in gastrocnemius infected with LncRNA-TBP overexpression (n = 4). B Relative glycogen content in LncRNA-TBP overexpression gastrocnemius (n = 5). C Relative mRNA expression levels of glycogenolytic and glycolytic genes in LncRNA-TBP overexpression gastrocnemius (n = 6). D Relative mRNA expression levels of several fast-/slow-twitch myofiber genes in overexpression of LncRNA-TBP gastrocnemius (n = 6). E and F Immunohistochemistry analysis of MYH1A/MYH7B (n = 3) (E) and MYH1A/MYH7B protein content (n = 8) (F) in gastrocnemius with LncRNA-TBP overexpression. G Relative gastrocnemius muscle weight after infected with lentivirus-mediated LncRNA-TBP overexpression (n = 6). H H&E staining in overexpression of LncRNA-TBP gastrocnemius (n = 3). I and J Relative mRNA (n = 6) (I) and the protein (n = 3) (J) expression levels of the autophagy-related genes in gastrocnemius with LncRNA-TBP overexpression. In panel J, the numbers shown below the bands were folds of band intensities relative to control. Band intensities were quantified by ImageJ and normalized to GAPDH. Data are expressed as a fold-change relative to the control. Results are shown as mean ± SEM. In panels A–D, F and H–I, the statistical significance of differences between means was assessed using an independent sample t-test. (*P < 0.05; **P < 0.01; N.S., no significant difference)