Fig. 3

LncRNA-TBP promotes fatty acid oxidation and TCA cycles in skeletal muscle. A Relative LncRNA-TBP expression in gastrocnemius after infected with lentivirus-mediated LncRNA-TBP overexpression (Lv-LncRNA-TBP) or negative control (Lv-NC) (n = 4). B Relative mtDNA content in LncRNA-TBP overexpression gastrocnemius (n = 4). C Relative fatty acid β-oxidation rate with overexpression of LncRNA-TBP in gastrocnemius (n = 4). D Relative free fatty acid (FFA) and triglyceride (TG) content in gastrocnemius with LncRNA-TBP overexpression (n = 4). E and F Relative mRNA (n = 6) (E) and protein (n = 3) (F) expression levels of fatty acid oxidation or synthesis related-genes in gastrocnemius with LncRNA-TBP overexpression. G Hierarchical clustering analysis (HCA) of metabolites in gastrocnemius with overexpression of LncRNA-TBP (n = 6). The colors indicate the relative levels in the overexpression of LncRNA-TBP or control group. H Relative metabolite content of glycolysis and tricarboxylic acid (TCA) cycle in gastrocnemius with LncRNA-TBP overexpression (n = 6). In panel F, the numbers shown below the bands were folds of band intensities relative to control. Band intensities were quantified by ImageJ and normalized to GAPDH. Data are expressed as a fold-change relative to the control. Results are shown as mean ± SEM. In panels (A–E and H), the statistical significance of differences between means was assessed using an independent sample t-test. (*P < 0.05; **P < 0.01; N.S., no significant difference)